Robert Buzzeo

Faculty

Clearwater Campus

Natural Science

NM 215

Buzzeo.Robert@SPCollege.edu

727-341-4423

Office Schedule: By Appointment


Course Info

Syllabus Links

Course syllabi are made available at the discretion of the faculty and may require you to log in; if a syllabus isn't accessible, the instructor may not have published it yet.

BSC4931 1072 0665

PCB3063 1162 0665

PCB4024 1191 0665

PCB3063L 1163 0665


Biography

Robert Buzzeo is a Professor of Biology at St. Petersburg College, where he teaches genetics, cell biology, molecular biology, biotechnology and senior seminar for biology students. He previously served as faculty at Hillsborough Community College and at the University of South Florida as both an instructor and research scientist in the Department of Cell Biology, Molecular Biology, and Microbiology. His instructional and laboratory experience includes tissue culture, flow cytometry, confocal microscopy, molecular biology, DNA sequencing, and transgenic model systems including Caenorhabditis elegans, Drosophila melanogaster, and Danio rerio.

Robert Buzzeo’s research and instructional interests has focused on cancer biology, cellular signaling pathways, drug resistance, and the molecular mechanisms underlying disease progression. He has extensive experience working with primary and drug-resistant cell lines, viral packaging systems, advanced imaging technologies, and genetic manipulation techniques. His work has also included studies in neurobiology, mitochondrial dysfunction, model organism genetics, and translational approaches to understanding cancer cell invasion and therapeutic resistance. 

Outside of academia, Robert enjoys spending time with his three sons and is an avid baseball fan. Go Rays! Go Titans!

ORCID

Selected Publications

  • Breedy S, Ratnayake WS, Lajmi L, Buzzeo R, Acevedo-Duncan M. PKC-ι/ζ signaling is crucial for invasiveness and apoptosis/pyroptosis inhibition of glioblastoma cells through upregulation of β-catenin, PDK1/Akt1 and Smad cascades via 14-3-3. Molecular Oncology. 2026. Manuscript in review.
  • Breedy S, Ratnayake WS, Lajmi L, Buzzeo R, Acevedo-Duncan M. 14-3-3 and Smad2/3 are crucial mediators of atypical-PKCs: Implications for neuroblastoma progression. Front Oncol. 2023;13:1051516. doi:10.3389/fonc.2023.1051516. PMID: 36776326. PMCID: PMC9910080.
  • Ratnayake WS, Apostolatos AH, Breedy S, Dennison CL, Buzzeo R, Acevedo-Duncan M. Atypical PKCs activate Vimentin to facilitate prostate cancer cell motility and invasion. Cell Adhes Migr. 2021;15(1):37–57. doi:10.1080/19336918.2021.1882782. PMID: 33525953. PMCID: PMC7889213.
  • Chhabra R, Rockfield S, Guergues J, Nadeau OW, Buzzeo R, Stevens SM Jr, Nanjundan M. Global miRNA/proteomic analyses identify miRNAs at 14q32 and 3p21, which contribute to features of chronic iron-exposed fallopian tube epithelial cells. Sci Rep. 2021;11(1):6270. doi:10.1038/s41598-021-85342-y. PMID: 33737539. PMCID: PMC7973504.
  • Apostolatos AH, Ratnayake WS, Win-Piazza H, Apostolatos CA, Smalley T, Kang L, Salup R, Buzzeo R, Acevedo-Duncan M. Inhibition of atypical protein kinase C-ι effectively reduces the malignancy of prostate cancer cells by downregulating the NF-κB signaling cascade. Int J Oncol. 2018;53(5):1836–1846. doi:10.3892/ijo.2018.4542. PMID: 30226591. PMCID: PMC6192717.
  • Falahat R, Berglund A, Putney RM, Perez-Villarroel P, Aoyama K, Pilon-Thomas S, Barber GN, Mulé JJ, Buzzeo R, Daefler S. A Cell ELISA for the quantification of MUC1 mucin (CD227) expressed by cancer cells of epithelial and neuroectodermal origin. Cell Immunol. 2015;298(1–2):96–103. doi:10.1016/j.cellimm.2015.09.006. PMID: 26435344.
  • Paullin TR, Rockfield S, Raffel J, Nguyen T, Martinez D, Goode T, Digman MA, Nanjundan M, Buzzeo R. Spheroid growth in ovarian cancer alters transcriptome responses for stress pathways and epigenetic responses. PLoS One. 2017;12(8):e0182930. doi:10.1371/journal.pone.0182930.
  • Tanguay RL, Andreasen E, Heideman W, Peterson RE, Buzzeo R. Functional characterization of aryl hydrocarbon receptor (zfAHR2) localization and degradation in zebrafish (Danio rerio). Biochem Pharmacol. 2004;67(7):1363–1372.
  • Alexandre PA, Kogelman LJ, Santana MH, Passarelli D, Pulz LH, Fantinato-Neto P, Silva PL, Leme PR, Strefezzi RF, Coutinho LL, Ferraz JB, Eler JP, Buzzeo R. Differential gene expression in the duodenum, jejunum and ileum among crossbred beef steers with divergent gain and feed intake phenotypes. Anim Genet. 2016;47(5):568–579. doi:10.1111/age.12440.
  • Teng P, Nimmagadda A, Su M, Li Y, Qiao Q, Shimamoto Y, Buzzeo R, Cao A, Cai J. Cellular translocation of a γ-AApeptide mimetic of Tat peptide. Mol Pharm. 2012;9(5):1529–1534. doi:10.1021/mp300070w.
  • Teng P, Qiao Q, Mowery BP, Shea C, Xin D, Shimamoto Y, Wang H-G, Buzzeo R, Cao A, Cai J. Cellular uptake of an α-AApeptide. Org Biomol Chem. 2012;10(6):1149–1153. doi:10.1039/C2OB06679C.
  • Chen J, Ye L, Zhang L, Jiang WG, Buzzeo R, Wang H, Kynaston H, Mason MD. Tipifarnib-induced apoptosis in acute myeloid leukemia and multiple myeloma cells depends on Ca2+ influx through plasma membrane Ca2+ channels. J Pharmacol Exp Ther. 2011;337(3):636–643. doi:10.1124/jpet.110.172809.
  • Dragicevic N, Copes N, O’Neal-Moffitt G, Jin J, Buzzeo R, Callaghan D, Delic V, Bradshaw PC, Dou QP, Mamcarz M, Zhang Y, Becker KG, Ehrlich ME, Sharfman WH, Albers DS, Nagele RG, Spencer RG, Chuang DM, Cai J, Kline AE, Brewer GJ, Wang X, Lahiri DK, Greig NH, Martin B, Mattson MP, Kapogiannis D. Melatonin treatment restores mitochondrial function in Alzheimer's mice: a mitochondrial protective role of melatonin membrane receptor signaling. J Pineal Res. 2011;51(1):75–86. doi:10.1111/j.1600-079X.2011.00864.x.
  • Dragicevic N, Mamcarz M, Zhu Y, Buzzeo R, Tan J, Arendash GW, Bradshaw PC. Mitochondrial amyloid-beta levels are associated with the extent of mitochondrial dysfunction in different brain regions and the degree of cognitive impairment in Alzheimer's transgenic mice. J Alzheimers Dis. 2010;20 Suppl 2:S535–S550. doi:10.3233/JAD-2010-100342.
  • David E, Sun SY, Waller EK, Chen J, Khuri FR, Chen DT, Moreb JS, Sebti SM, Hamilton AD, Dalton WS, Sebti SM, Buzzeo R, Alsina M. Tipifarnib and bortezomib are synergistic and overcome cell adhesion-mediated drug resistance in multiple myeloma and acute myeloid leukemia. Clin Cancer Res. 2006;12(2):591–599.
  • David E, Ecsedy JA, Sun SY, Zhou W, Mims MP, Khuri FR, Mita AC, Chandra J, Buzzeo R, Wang H-G, Grant S, Sebti SM, Dalton WS. Characterization of a R115777-resistant human multiple myeloma cell line with cross-resistance to PS-341. Clin Cancer Res. 2005;11(16):6057–6064.

Additional Work



Edit Instructor